Journal: JHEP Reports

Article Title: Placental growth factor modulates endothelial NO production and exacerbates experimental hepatopulmonary syndrome

doi: 10.1016/j.jhepr.2024.101297

Figure Lengend Snippet: HPS development in Plgf –/– rats after CBDL and PPVL surgery. (A) Experimental procedures. (B) Circulating PlGF levels in serum determined using ELISA. (C) Detection of Plgf mRNA levels in liver and lung using RT-qPCR. (D) A-aO 2 in arterial blood. (E) Quantification of contrast-enhanced transthoracic echocardiogram: ratio of the difference in acoustic signal between baseline and after injection in the left ventricle to the right ventricle. (F) Quantification of total microspheres in one kidney by fluorescence-activated cell sorting after jugular vein injection. Data are mean ± SEM; comparisons were made using one-way ANOVA followed by the Tukey test: n.s., not significant ( p >0.05); ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001, vs. Sham WT group; &&& p <0.001, &&&& p <0.0001 vs. WT CBDL. A-aO 2 , alveolar–arterial oxygen gradient; CBDL, common bile duct ligation; HPS, hepatopulmonary syndrome; PIGF, placental growth factor; PPVL, partial portal vein ligation; real-time quantitative PCR (RT-qPCR); WT, wild-type.

Article Snippet: Serum PlGF levels were quantified using an ELISA kit (E-CL-R0520, ElabScience, Houston, Texas, 77079, USA) following the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Injection, Fluorescence, FACS, Ligation, Real-time Polymerase Chain Reaction

Journal: Frontiers in Oncology

Article Title: Multi-platform Affinity Proteomics Identify Proteins Linked to Metastasis and Immune Suppression in Ovarian Cancer Plasma

doi: 10.3389/fonc.2019.01150

Figure Lengend Snippet: Cellular origin of upregulated proteins. (A) Transcriptome analysis of the top 30 proteins increased in OC-plasma (from ). (B) Proteome analysis as in (A) . Due to the lower sensitivity of MS-based proteomics, especially for secreted proteins , data were not available for a number of proteins. Boxplots show medians (horizontal line in boxes), upper and lower quartiles (box) and range (whiskers). Arrows point out MUC16, SPINT2, and WFDC2. TU, tumor cells; TAM, tumor-associated macrophage; TAT, tumor-associated T-cells.

Article Snippet: Other proteins were quantified by ELISA according to the instructions of the respective manufacturer: BCAM (ELH-BCAM-2; BioCat GmbH, Heidelberg, Germany); EPHA2 (ELH-EPHA2-1; RayBiotech Life, Peachtree Corners, GA, USA); GDF15 (DGD150; R&D Systems, Wiesbaden, Germany); IL-6 (Invitrogen-88-7066-22; Thermo Fisher Scientific, Schwerte, Germany); IL-18BP (DBP180; R&D Systems, Wiesbaden, Germany); OPN/SPP1 (DOST00; R&D Systems, Wiesbaden, Germany); SPON1 (CSB-EL022599HU-96; Cusabio, Houston, TX, USA); VEGFA (BMS277-2; Thermo Fisher Scientific, Schwerte, Germany); WFDC2/HE4 (DHE400; R&D Systems, Wiesbaden, Germany); SPINT2 (EK0773-CAP; Boster, Pleasanton, USA); PVRL4/NECTIN4 (DNEC40; R&D Systems, Wiesbaden, Germany).

Techniques: Clinical Proteomics

Journal: Frontiers in Oncology

Article Title: Multi-platform Affinity Proteomics Identify Proteins Linked to Metastasis and Immune Suppression in Ovarian Cancer Plasma

doi: 10.3389/fonc.2019.01150

Figure Lengend Snippet: Association of SPINT2 mRNA expression with survival of OC patients. (A) Kaplan-Meier plot for 1074 HGSC patients in the Kaplan–Meier Plotter database (updated version at http://kmplot.com ) analyzing the association of SPINT2 with RFS. HR, hazard ratio. (B) Kaplan-Meier plot for 1074 HGSC patients in the same database analyzing the association of SPINT2 with OS. (C) z-scores (PRECOG data) for the association of SPINT2 with the overall survival (OS) of the indicated tumor entities . Red: association with a short OS (z-score above +1.5;). Blue: association with a short OS (z-score below −1.5).

Article Snippet: Other proteins were quantified by ELISA according to the instructions of the respective manufacturer: BCAM (ELH-BCAM-2; BioCat GmbH, Heidelberg, Germany); EPHA2 (ELH-EPHA2-1; RayBiotech Life, Peachtree Corners, GA, USA); GDF15 (DGD150; R&D Systems, Wiesbaden, Germany); IL-6 (Invitrogen-88-7066-22; Thermo Fisher Scientific, Schwerte, Germany); IL-18BP (DBP180; R&D Systems, Wiesbaden, Germany); OPN/SPP1 (DOST00; R&D Systems, Wiesbaden, Germany); SPON1 (CSB-EL022599HU-96; Cusabio, Houston, TX, USA); VEGFA (BMS277-2; Thermo Fisher Scientific, Schwerte, Germany); WFDC2/HE4 (DHE400; R&D Systems, Wiesbaden, Germany); SPINT2 (EK0773-CAP; Boster, Pleasanton, USA); PVRL4/NECTIN4 (DNEC40; R&D Systems, Wiesbaden, Germany).

Techniques: Expressing

Journal: Molecular human reproduction

Article Title: Decreased expression of NR4A nuclear receptors in adenomyosis impairs endometrial decidualization.

doi: 10.1093/molehr/gaw042

Figure Lengend Snippet: Figure 2 NR4A regulation of human endometrial stromal cell (hESC) decidualization in vitro. hESCs were transduced with Ad-LacZ or Ad-NR4A at a multiplicity of infection (MOI) of 25 or were treated with 0.5 mM 8-Br-cAMP and 1 µM MPA for 48 h. PRL (A) and IGFBP1 (B) mRNA levels were measured by real-time PCR. The results are presented as the mean ± SEM; n = 3 samples. * P < 0.05, ** P < 0.01, and *** P < 0.001 compared with Ad-LacZ. hESCs were infected with the indicated adenoviruses at a MOI of 50, followed by treatment with or without 8-Br-cAMP and MPA for an add- itional 4 days. Prolactin (C) and IGFBP-1 (D) released into the medium was measured by Enzyme Linked Fluorescent Assay (ELFA) and ELISA. The results are presented as the mean ± SEM; n = 5 samples. * P < 0.05, ** P < 0.01, and *** P < 0.001 compared with Ad-LacZ. hESCs were transfected with 50 nM of the indicated specific NR4A siRNA for 72 h, followed by treatment with or without 8-Br-cAMP and MPA for an additional 4 days. Prolactin (E) and IGFBP-1 (F) released into the medium were measured by ELFA and ELISA. The results are presented as the mean ± SEM; n = 4 sam- ples. *** P < 0.001 compared with siCTL. # P < 0.05 and ## P < 0.01 compared with siCTL plus 8-Br-cAMP and MPA. (G and H) Fluorescein isothio- cyanate labeled phalloidin was used to label actin filaments, and immunofluorescence was used to analyze the morphological transformation of hESCs.

Article Snippet: Detection of prolactin and IGFBP-1 levels in cultured supernatants Prolactin and IGFBP-1 levels in supernatants were measured using a Vidas prolactin kit (bioMérieux) and a human IGFBP1 ELISA kit (BOSTER).

Techniques: In Vitro, Transduction, Infection, Real-time Polymerase Chain Reaction, Fluorescence, Enzyme-linked Immunosorbent Assay, Transfection, Labeling, Transformation Assay